Coronavirus Tuntun (SARS-Cov-2) Ohun elo Iwari Acid Nucleic
New Coronavirus(SARS-Cov-2) Nucleic Acid Detection Kit
(Fluirinscent RT-PCR Probe Method) Product Manual
【Product name 】Coronavirus Tuntun (SARS-Cov-2) Apo Iwari Acid Nucleic (Ọna Iwadi RT-PCR Fluorescent)
【Packaging specifications 】25 Idanwo / Kit
【Intended usọjọ ori】
A lo ohun elo yii fun wiwa ti agbara ti acid nucleic lati inu coronavirus tuntun ni awọn swabs nasopharyngeal, swabs oropharyngeal (ọfun), swabs iwaju iwaju, swabs aarin-turbinate, awọn iwẹ imu ati awọn aspirates imu lati ọdọ awọn ẹni-kọọkan ti wọn fura si COVID19 nipasẹ olupese ilera wọn. Wiwa ti ORF1ab ati awọn Jiini N ti coronavirus tuntun le ṣee lo fun ayẹwo iranlọwọ ati ibojuwo ajakale-arun ti ikolu coronavirus tuntun.
【Principles of the procedure 】
Ohun elo yii jẹ apẹrẹ fun awọn iwadii TaqMan kan pato ati awọn alakoko kan pato ti a ṣe apẹrẹ fun aramada coronavirus (SARS-Cov-2) ORF1ab ati awọn ilana Jiini N. Ojutu idahun PCR ni awọn eto 3 ti awọn alakoko kan pato ati awọn iwadii fluorescent fun wiwa kan pato ti awọn ibi-afẹde, ati eto afikun ti awọn alakoko kan pato ati awọn iwadii Fuluorisenti ni a lo bi iṣakoso boṣewa inu ti ohun elo fun wiwa awọn jiini itọju ile endogenous.
Ilana idanwo naa ni pe iwadii fluorescent kan pato ti wa ni digested ati ibajẹ nipasẹ iṣẹ exonuclease ti Taq henensiamu ninu iṣesi PCR, ki ẹgbẹ fluorescent ti onirohin ati ẹgbẹ fluorescent ti o ti pa ti yapa, ki eto ibojuwo fluorescence le gba fluorescent kan. ifihan agbara, ati lẹhinna Nipasẹ ipa imudara ti PCR ampilifaya, ifihan agbara fluorescence ti iwadii naa de iye ala ti a ṣeto-iye Ct (Ilana Yiyi). Ni ọran ti ko si amplicon ibi-afẹde, ẹgbẹ onirohin ti iwadii wa nitosi ẹgbẹ ti o pa. Ni akoko yii, iṣipopada agbara fifẹ fluorescence waye, ati fifẹ ti ẹgbẹ onirohin ti parun nipasẹ ẹgbẹ quenching, ki ifihan agbara fluorescent ko le ṣee wa-ri nipasẹ ohun elo PCR fluorescent.
Lati le ṣe atẹle lilo awọn reagents lakoko idanwo naa, ohun elo naa ni ipese pẹlu awọn iṣakoso rere ati odi: iṣakoso rere ni aaye ibi-afẹde recombinant plasmid, ati iṣakoso odi jẹ omi Distilled, eyiti o lo lati ṣe atẹle idoti ayika. A ṣe iṣeduro lati ṣeto iṣakoso rere ati iṣakoso odi nigbakanna nigba idanwo.
【Main components 】
| Cat. No. | BST-SARS-25 | BST-SARS-DR-25 | Kopọents | |
| Name | Specification | Quantity | Quantity | |
| Iṣakoso to dara | 180 μL / vial | 1 | 1 | Awọn plasmids ti a ṣe ni atọwọdọwọ, omi Distilled |
| Iṣakoso odi | 180 μL / vial | 1 | 1 | Distilled omi |
| SARS-Cov-2 Mix | 358,5 μL / vial | 1 | / | Awọn orisii alakoko kan pato, wiwa kan pato awọn iwadii fluorescent, dNTPs, , MgCl2, KCl, Tris-Hcl, omi distilled, bbl |
| Enzymu Mix | 16,5 μL / vial | 1 | / | Awọn enzymu Taq, iyipada transcriptase, awọn enzymu UNG, ati bẹbẹ lọ. |
| Apapo SARS-Cov-2 (Lyophilized) | 25 igbeyewo / vial | / | 1 | Awọn orisii alakoko kan pato, awọn iwadii fluorescent kan pato, awọn dNTPs, awọn ensaemusi Taq, iyipada transcriptase, omi Distilled, ati bẹbẹ lọ. |
| 2x Ifipamọ | 375 μL/vial | / | 1 | MgCl2, KCl, Tris-Hcl, Distilled omi, ati be be lo. |
Akiyesi:( 1) Awọn paati ti o wa ninu awọn ohun elo ipele oriṣiriṣi ko le dapọ tabi paarọ.
(2) Mura reagent tirẹ: Ohun elo isediwon acid Nucleic.
【Storage conditions ati expiration date 】
For BST-SARS-25:Ọkọ ati fipamọ ni -20 ± 5 ℃ fun igba pipẹ.
For BST-SARS-DR-25:Gbigbe ni iwọn otutu yara. Fipamọ ni -20± 5℃ fun igba pipẹ.
Yago fun tun di-thaw iyika. Akoko wiwulo ti ṣeto fun awọn oṣu 12.
Wo aami fun ọjọ iṣelọpọ ati lilo.
Lẹhin ṣiṣi akọkọ, reagent le wa ni ipamọ ni -20 ± 5 ° C fun ko ju oṣu 1 lọ tabi titi di opin akoko reagent, eyikeyi ọjọ ti o wa ni akọkọ, lati yago fun awọn akoko didi-diẹ leralera, ati nọmba ti didi reagent. -thaw cycles yẹ ki o ko koja 6 igba.
【Applicable instrument】ABI 7500, SLAN-96P, Roche-LightCycler-480.
【Sample requirements 】
1.Aṣamulo iru apẹẹrẹ: Ti a jade kuro ni ojutu nucleic acid.
2.Sample ipamọ ati gbigbe: Fipamọ ni-20 ± 5 ℃ fun awọn osu 6. Dii ati ki o yọ awọn ayẹwo ko ju awọn akoko 6 lọ.
【Testing method】
1.Nucleic acid extraction
Yan ohun elo isediwon acid nucleic ti o yẹ lati yọkuro acid nucleic ti gbogun, ki o tẹle awọn ilana kit ti o baamu. A gba ọ niyanju lati lo isediwon acid Nucleic ati ohun elo ìwẹnumọ ti Yixin Bio-Tech (Guangzhou) Co., Ltd. tabi ohun elo isọdọmọ acid nucleic deede.
2. Reaction reagent preparation
2.1 For BST-SARS-25:
( 1) Yọ SARS-Cov-2 Mix ati Enzyme Mix, yo patapata ni iwọn otutu yara daradara nipasẹ ẹrọ Vortex ati lẹhinna centrifuge ni ṣoki.
(2) 16.5uL Enzyme Mix ni a ṣafikun si 358.5uL SARS-Cov-2 Mix ati dapọ daradara lati gba ojutu ifapapọ.
(3) Mura 0.2 mL PCR octal tube ti o mọ ki o samisi pẹlu 15uL ti ojutu ifaseyin adalu loke fun kanga.
(4) Ṣafikun 15 μL ti ojutu nucleic acid mimọ, iṣakoso rere ati iṣakoso odi, ati farabalẹ bo fila tube octal.
(5) Darapọ daradara nipa yiyipada lodindi, ati ni kiakia centrifuge lati ṣojumọ omi ni isalẹ ti tube.
1
2.2 For BST-SARS-DR-25:
( 1) Ṣafikun 375ul 2x Buffer si SARS-Cov-2 Mix ((Lyophilised) lati ṣeto apopọ ifaseyin. Darapọ daradara nipasẹ pipetting ati lẹhinna centrifuge ni ṣoki. ipamọ igba pipẹ.)
(2) Mura 0.2 mL PCR octal tube ti o mọ ki o samisi pẹlu 15μL ti idapọ ifaseyin fun kanga.
(3) Ṣafikun 15μL ti ojutu nucleic acid mimọ, iṣakoso rere ati iṣakoso odi, ati farabalẹ bo fila tube octal.
(4) Illa daradara nipa yiyipada lodindi, ati ni kiakia centrifuge lati koju omi ni isalẹ ti tube.
3. PCR amplification (Jọwọ tọka si iwe ilana irinṣẹ fun awọn eto iṣẹ.)
3. 1 Gbe PCR 8-tube sinu iyẹwu ayẹwo ti ohun elo PCR fluorescent, ki o si ṣeto ayẹwo lati ṣe idanwo, iṣakoso rere ati iṣakoso odi gẹgẹbi aṣẹ ti ikojọpọ.
3.2 ikanni wiwa Fluorescence:
( 1) ORF1ab pupọ yan ikanni wiwa ti FAM (Orohin: FAM, Quencher: Ko si).
(2) N jiini yan ikanni wiwa ti VIC (Orohin: VIC, Quencher: Ko si).
(3) Jiini boṣewa inu yan ikanni wiwa ti CY5 (Orohin: CY5, Quencher: Ko si).
(4) Itọkasi Palolo ti ṣeto si ROX.
Eto paramita eto 3.3 PCR:
| Igbesẹ | Iwọn otutu (℃) | Akoko | Nọmba ti iyika | |
| 1 | Idahun transcription yiyipada | 50 | 15 min | 1 |
| 2 | Mu ṣiṣẹ enzymu Taq | 95 | 2.5 iṣẹju | 1 |
| 3 | Mu ṣiṣẹ enzymu Taq | 93 | 10 iṣẹju-aaya | 43 |
| Annealing itẹsiwaju ati fluorescence akomora | 55 | 30 iṣẹju-aaya | ||
Lẹhin ti ṣeto, ṣafipamọ faili naa ki o ṣiṣẹ eto ifura naa..
4.Results analysis
Lẹhin ti eto naa pari, awọn abajade ti wa ni fipamọ laifọwọyi, ati pe a ti ṣe atupale ohun-elo imudara. Ipilẹ imudara ti ṣeto si iloro aiyipada irinse.
【Explanation of test results 】
1. Ṣe ipinnu iwulo ti ṣàdánwò: FAM iṣakoso rere, ikanni VIC yẹ ki o ni ọna imudara aṣoju, ati pe iye Ct ko kere ju 34, ṣugbọn o le yipada nitori awọn eto iloro oriṣiriṣi ti awọn ohun elo oriṣiriṣi. FAM iṣakoso odi, ikanni VIC yẹ ki o jẹ ti kii ṣe imudara Ct. O gba pe awọn ibeere ti o wa loke gbọdọ pade ni akoko kanna, bibẹẹkọ idanwo yii ko wulo.
2. Idajọ esi
| FAM / VIC ikanni | Abajade idajo |
| Ct;37 | Idanwo ayẹwo jẹ rere |
| 37≤CT40 | Iwọn imudara jẹ apẹrẹ S, ati awọn ayẹwo ifura nilo lati tun ṣe ayẹwo; ti awọn abajade atunyẹwo tun wa ni ibamu, a ṣe idajọ rẹ bi rere, bibẹẹkọ o jẹ odi |
| Ct≥40 Tabi Ko si Imudara | Idanwo ayẹwo jẹ odi (tabi isalẹ opin opin ti wiwa ohun elo) |
Akiyesi: ( 1) Ti ikanni FAM mejeeji ati ikanni VIC ba ni idaniloju ni akoko kanna, SARS-Cov-2 ti pinnu lati jẹ rere.
(2) Ti ikanni FAM tabi ikanni VIC ba jẹ rere ati pe ikanni miiran jẹ odi, idanwo naa yẹ ki o tun ṣe. Ti o ba jẹ rere ni akoko kanna, yoo ṣe idajọ bi SARS-Cov-2 rere, bibẹẹkọ yoo ṣe idajọ bi odi SARS-Cov-2.
Awọn ọja ti o jọmọ
-
Apo Iwari Antibody COVID-19 IgM/IgG
Apo Iwari Antibody COVID-19 IgM/IgG (Ọna Colloidal Gold Immunochromatography) Afọwọṣe Ọja 【ORUKO Ọja】COVID-19 IgM/IgG Apo Iwari Antibody (Ọna Ajẹsara Gold Colloidal Immunochromatography)【KỌRỌ IṢẸYỌRỌ/Awọn Idanwo1 ABSTRACT】 Awọn aramada coronaviruses jẹ ti iwin β. COVID-19 jẹ arun aarun atẹgun nla kan. Awọn eniyan ni ifaragba gbogbogbo. Lọwọlọwọ, awọn alaisan ti o ni arun coronavirus aramada jẹ orisun akọkọ…
-
Iyọkuro Acid Nucleic Tabi Apo Iwẹnumọ
Iyọkuro Acid Nucleic Tabi Apo Iwẹnumọ tabi ti a fipamọ si -20℃. Ayẹwo yẹ ki o wa ni gbigbe ni lilo 0 ℃ curling. Ibẹrẹ Iyọkuro Acid Nucleic Acid Tabi Apo Isọdipo (Ọna Awọn Ilẹkẹ Oofa) jẹ apẹrẹ fun isọdinu adaṣe adaṣe ti RNA ati DNA lati awọn omi ara (bii swabs, pilasima, omi ara) ni lilo awọn ohun elo isediwon acid nucleic adaṣe. Imọ-ẹrọ patiku oofa n pese DNA/RNA ti o ni agbara ti o dara fun ...
-
SARS-CoV-2 Antigen Assay Kit (Immunochromatogr…
SARS-CoV-2 Antigen Assay Kit (Ọna Immunochromatography) Ọja Afowoyi 【orukọ ọja】SARS-CoV-2 Antigen Assay Apo (Ọna Immunochromatography) 【PACKAGING SECIFICATIONS】 1 Idanwo/Apo 【ABSTRACT】 Novel naa jẹ ti coronavirus. COVID-19 jẹ arun aarun atẹgun nla kan. Awọn eniyan ni ifaragba gbogbogbo. Lọwọlọwọ, awọn alaisan ti o ni arun coronavirus aramada jẹ akọkọ…
-
SARS-CoV-2 Antigen Assay Kit
SARS-CoV-2 Antigen Assay Kit (Ọna Immunochromatography) Ọja Afowoyi 【ORUKO Ọja】SARS-CoV-2 Antigen Assay Kit (Ọna Imunochromatography) 【PACKAGING SECIFICATIONS】 1 Idanwo/Apo, 25Tests/Kit, 100 aramada coronaviruses jẹ ti iwin β. COVID-19 jẹ arun aarun atẹgun nla kan. Awọn eniyan ni ifaragba gbogbogbo. Lọwọlọwọ, awọn alaisan ti o ni arun coronavirus aramada jẹ orisun akọkọ ti ikolu; eniyan ti o ni asymptomatic...